insertional mutagenesis การใช้

• At first, T-DNA was applied for insertional mutagenesis.
• This is advantageous as insertional mutagenesis is avoided.
• The SB transposon is a powerful tool for insertional mutagenesis in many vertebrate species.
• Because of that property, transposons have been manipulated for use in insertional mutagenesis.
• In 1994, a new allele of reeler was obtained by means of insertional mutagenesis.
• Currently transposons can be used in genetic research and recombinant genetic engineering for insertional mutagenesis.
• Insertional mutagenesis is when transposons function as vectors to help remove and integrate genetic sequences.
• Avian leukosis virus is an example of a virus that causes a disease by insertional mutagenesis.
• An alternative strategy for insertional mutagenesis has been used in vertebrate animals to find genes that cause cancer.
• There is concern that insertional mutagenesis due to integration into the host genome might lead to cancer or leukemia.
• This is desirable in case the recombinant gene causes a mutation leading to uncontrolled cell growth ( insertional mutagenesis ).
• In mice, a dysfunctional " MAOA " gene is created through insertional mutagenesis ( called  Tg8 ).
• This gives rise to a concern that insertional mutagenesis due to integration into the host genome might lead to cancer or leukemia.
• Virus insertional mutagenesis is possible with both replication competent virus and the self-inactivating vectors that are commonly used in gene therapy.
• This condition is known as insertional mutagenesis, where the random insertion of a gene interferes with the tumor suppressor gene or stimulates an oncogene.
• With this approach there is increased risk of insertional mutagenesis; however, the risk can be reduced by using an integrase-deficient lentivirus.
• There is an example of an insertional mutagenesis event caused by a retrotransposon in the human genome where it causes Fukuyama-type muscular dystrophy.
• Specific databases for model animals include the Retrovirus Tagged Cancer Gene Database ( RTCGD ) that compiled research on retroviral and transposon insertional mutagenesis in mouse tumors.
• To date, four children in the French trial and one in the British trial have developed leukemia as a result of insertional mutagenesis by the retroviral vector.
• In an effort to help maximize the utility of the zebrafish as a model organism, Hopkins and colleagues set out to develop a large-scale insertional mutagenesis method.